human lung cancer cells h358 Search Results


human non-small lung cancer cell nci-h358  (China Center for Type Culture Collection)
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China Center for Type Culture Collection human non-small lung cancer cell nci-h358
APS inhibits <t>A549</t> and NCI-H358 cell growth in a concentration- and time-dependent manner. A549 cells were treated with different concentration of APS for 24 h a and with 20 mg/mL APS for different time b , NCI-H358 cells were treated with different concentration of APS for 24 h c and with 20 mg/mL APS for different time d . The growth inhibition of 20 mg/mL APS on A549 and NCI-H358 cells with or without the 12 h pre-incubation of the NF-κB activator PMA or NF-κB inhibitor Bay was shown in e , f respectively. Representative data of three independent experiments are shown
Human Non Small Lung Cancer Cell Nci H358, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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APS inhibits A549 and NCI-H358 cell growth in a concentration- and time-dependent manner. A549 cells were treated with different concentration of APS for 24 h a and with 20 mg/mL APS for different time b , NCI-H358 cells were treated with different concentration of APS for 24 h c and with 20 mg/mL APS for different time d . The growth inhibition of 20 mg/mL APS on A549 and NCI-H358 cells with or without the 12 h pre-incubation of the NF-κB activator PMA or NF-κB inhibitor Bay was shown in e , f respectively. Representative data of three independent experiments are shown

Journal: Cancer Cell International

Article Title: Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells

doi: 10.1186/s12935-017-0487-6

Figure Lengend Snippet: APS inhibits A549 and NCI-H358 cell growth in a concentration- and time-dependent manner. A549 cells were treated with different concentration of APS for 24 h a and with 20 mg/mL APS for different time b , NCI-H358 cells were treated with different concentration of APS for 24 h c and with 20 mg/mL APS for different time d . The growth inhibition of 20 mg/mL APS on A549 and NCI-H358 cells with or without the 12 h pre-incubation of the NF-κB activator PMA or NF-κB inhibitor Bay was shown in e , f respectively. Representative data of three independent experiments are shown

Article Snippet: Human non-small lung cancer cell A549 and NCI-H358 were purchased from CCTCC (China Center for Type Culture Collection, Wuhan, China).

Techniques: Concentration Assay, Inhibition, Incubation

APS delays the growth of A549 xenograft in BALB/C nude mice. A549 lung adenocarcinoma cells (5 × 10 6 ) were subcutaneously inoculated in the upper left flank of mice, the mice were randomly divided into three group (n = 8 each): Negative control (treated with normal saline), APS (300 mg/kg per day for 30 days through i.p injection), and positive control (8 mg/kg DDP once a week for 3 weeks). Tumor size was recorded every 3 days and the volume was calculated using the following formula: (L × W 2 )/2, where L equals length and W equals width

Journal: Cancer Cell International

Article Title: Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells

doi: 10.1186/s12935-017-0487-6

Figure Lengend Snippet: APS delays the growth of A549 xenograft in BALB/C nude mice. A549 lung adenocarcinoma cells (5 × 10 6 ) were subcutaneously inoculated in the upper left flank of mice, the mice were randomly divided into three group (n = 8 each): Negative control (treated with normal saline), APS (300 mg/kg per day for 30 days through i.p injection), and positive control (8 mg/kg DDP once a week for 3 weeks). Tumor size was recorded every 3 days and the volume was calculated using the following formula: (L × W 2 )/2, where L equals length and W equals width

Article Snippet: Human non-small lung cancer cell A549 and NCI-H358 were purchased from CCTCC (China Center for Type Culture Collection, Wuhan, China).

Techniques: Negative Control, Saline, Injection, Positive Control

The downregulation of APS on p65 and p50 expression in A549 cells. The RT-PCR (reverse transcription PCR) and Western blot were applied to measure the expression level of p65 mRNA and protein a , b . Western blot was applied to measure the expression level of p50 protein in nuclei c . Representative data of three independent experiments are shown

Journal: Cancer Cell International

Article Title: Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells

doi: 10.1186/s12935-017-0487-6

Figure Lengend Snippet: The downregulation of APS on p65 and p50 expression in A549 cells. The RT-PCR (reverse transcription PCR) and Western blot were applied to measure the expression level of p65 mRNA and protein a , b . Western blot was applied to measure the expression level of p50 protein in nuclei c . Representative data of three independent experiments are shown

Article Snippet: Human non-small lung cancer cell A549 and NCI-H358 were purchased from CCTCC (China Center for Type Culture Collection, Wuhan, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Western Blot

The inhibtion of APS on NF-κB transcription activity in A549 cells. The NF-κB-luciferase plasmid construct (pNF-κB-Luc) was used to study NF-κB activity. pNF-κB-Luc were transfected into the A549 cells, the cells then were treated with APS and DNSO for 6 h. The luciferase activity of NF-κB was determined according the protocol. Representative data of three independent experiments are shown

Journal: Cancer Cell International

Article Title: Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells

doi: 10.1186/s12935-017-0487-6

Figure Lengend Snippet: The inhibtion of APS on NF-κB transcription activity in A549 cells. The NF-κB-luciferase plasmid construct (pNF-κB-Luc) was used to study NF-κB activity. pNF-κB-Luc were transfected into the A549 cells, the cells then were treated with APS and DNSO for 6 h. The luciferase activity of NF-κB was determined according the protocol. Representative data of three independent experiments are shown

Article Snippet: Human non-small lung cancer cell A549 and NCI-H358 were purchased from CCTCC (China Center for Type Culture Collection, Wuhan, China).

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Construct, Transfection

The influence of APS on CyclinD1 and Bcl-xL protein expression in A549 cells. The Western blot were applied to measure the expression level of CyclinD1 a and Bcl-xL b protein. Representative data of three independent experiments are shown

Journal: Cancer Cell International

Article Title: Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells

doi: 10.1186/s12935-017-0487-6

Figure Lengend Snippet: The influence of APS on CyclinD1 and Bcl-xL protein expression in A549 cells. The Western blot were applied to measure the expression level of CyclinD1 a and Bcl-xL b protein. Representative data of three independent experiments are shown

Article Snippet: Human non-small lung cancer cell A549 and NCI-H358 were purchased from CCTCC (China Center for Type Culture Collection, Wuhan, China).

Techniques: Expressing, Western Blot